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首页> 外文OA文献 >O-Glycosylation of Axl2/Bud10p by Pmt4p Is Required for Its Stability, Localization, and Function in Daughter Cells
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O-Glycosylation of Axl2/Bud10p by Pmt4p Is Required for Its Stability, Localization, and Function in Daughter Cells

机译：通过Pmt4p对Axl2 / Bud10p进行O-糖基化是其在子细胞中的稳定性，定位和功能所必需的

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摘要

Cells of the yeast Saccharomyces cerevisiae choose bud sites in a manner that is dependent upon cell type: a and α cells select axial sites; a/α cells utilize bipolar sites. Mutants specifically defective in axial budding were isolated from an α strain using pseudohyphal growth as an assay. We found that a and α mutants defective in the previously identified PMT4 gene exhibit unipolar, rather than axial budding: mother cells choose axial bud sites, but daughter cells do not. PMT4 encodes a protein mannosyl transferase (pmt) required for O-linked glycosylation of some secretory and cell surface proteins (Immervoll, T., M. Gentzsch, and W. Tanner. 1995. Yeast. 11:1345–1351). We demonstrate that Axl2/Bud10p, which is required for the axial budding pattern, is an O-linked glycoprotein and is incompletely glycosylated, unstable, and mislocalized in cells lacking PMT4. Overexpression of AXL2 can partially restore proper bud-site selection to pmt4 mutants. These data indicate that Axl2/Bud10p is glycosylated by Pmt4p and that O-linked glycosylation increases Axl2/ Bud10p activity in daughter cells, apparently by enhancing its stability and promoting its localization to the plasma membrane.
机译：酵母酿酒酵母的细胞以取决于细胞类型的方式选择芽位点：a和α细胞选择轴向位点； a /α细胞利用双极位点。使用假菌丝生长作为测定，从α菌株中分离出在轴向出芽中特异缺陷的突变体。我们发现在先前确定的PMT4基因中有缺陷的a和α突变体表现出单极而不是轴向出芽：母细胞选择了轴向芽位，而子代细胞则没有。 PMT4编码一种甘露糖基转移酶（pmt），用于某些分泌和细胞表面蛋白的O型糖基化（Immervoll，T.，M.Gentzsch，and W.Tanner。1995. Yeast。11：1345-1351）。我们证明轴向出芽模式所需的Axl2 / Bud10p是一个O联糖蛋白，在缺少PMT4的细胞中不完全糖基化，不稳定和错位。 AXL2的过表达可以部分恢复pmt4突变体的正确芽位选择。这些数据表明Axl2 / Bud10p被Pmt4p糖基化，而O联糖基化显然通过增强其稳定性并促进其定位于质膜而增加了子细胞中Axl2 / Bud10p的活性。

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